Dr Laura Sharpe

Research Associate

PhD 2011

Science

School of Biotech & Biomolecular Science

I am a part-time Post-Doctoral Research Associate, in 's Lab in the School of BABS. I have been working at UNSW since 2011.

My work investigates the regulation of cholesterol synthesis enzymes, with a particular focus on enzymes that are marked for degradation by the E3 ubiquitin ligase, MARCHF6.

E-mail

laura@unsw.edu.au

Location

Level 3, Biological Sciences South E26

Book Chapters | 2021

Brown AJ; Coates HW; Sharpe LJ, 2021, 'Cholesterol synthesis', in Biochemistry of Lipids, Lipoproteins and Membranes, Elsevier, pp. 317 - 355,

Book Chapters | 2021

Brown AJ; Sharpe LJ, 2021, 'Oxysterols', in Encyclopedia of Molecular Pharmacology, Springer International Publishing, pp. 1198 - 1203,

Book Chapters | 2016

Brown AJ; Sharpe LJ, 2016, 'Chapter 11 Cholesterol Synthesis', in Biochemistry of Lipids, Lipoproteins and Membranes, Elsevier, pp. 327 - 358,

Book Chapters | 2016

Brown AJ; Sharpe LJ, 2016, 'Cholesterol Synthesis', in Biochemistry of Lipids, Lipoproteins and Membranes: Sixth Edition, pp. 327 - 358,

Book Chapters | 2015

Sharpe LJ; Brown AJ, 2015, 'Investigating Signaling Processes in Membrane Trafficking', in Methods in Molecular Biology, Springer New York, pp. 81 - 90,

Book Chapters | 2015

Sharpe LJ; Brown AJ, 2015, 'Investigating signaling processes in membrane trafficking.', in Membrane Trafficking, Springer, New York, pp. 81 - 90,

Book Chapters | 2008

Sharpe LJ; Du XR; Brown AJ, 2008, 'Approaches to investigate the role of signaling in ER-to-Golgi transport', in Sheehan D; Tyther R (ed.), Methods in Molecular Biology, Two Dimensional Electrophoresis Protocols, Humana Press, Inc., United States, pp. 215 - 227
Journal articles | 2024

Fenton NM; Qian L; Scott NA; Paine EG; Sharpe LJ; Brown AJ, 2024, 'SC5D is the sixth enzyme in cholesterol biosynthesis targeted by the E3 ubiquitin ligase MARCHF6', Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1869,

Journal articles | 2023

Fenton NM; Nguyen TB; Sharpe LJ; Brown AJ, 2023, 'Refining sugar's involvement in cholesterol synthesis', Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1868,

Journal articles | 2023

Qian L; Scott NA; Capell-Hattam IM; Draper EA; Fenton NM; Luu W; Sharpe LJ; Brown AJ, 2023, 'Cholesterol synthesis enzyme SC4MOL is fine-tuned by sterols and targeted for degradation by the E3 ligase MARCHF6', Journal of Lipid Research, 64,

Journal articles | 2023

Qian L; Sharpe LJ; Gokool A; Voineagu I; Brown AJ, 2023, 'Controlling an E3 ligase and its substrate: A function for MARCHF6 circRNA', Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1868,

Journal articles | 2022

Capell-Hattam IM; Fenton NM; Coates HW; Sharpe LJ; Brown AJ, 2022, 'The Non Catalytic Protein ERG28 has a Functional Role in Cholesterol Synthesis and is Coregulated Transcriptionally', Journal of Lipid Research, 63,

Journal articles | 2022

Yang A; Alrosan AZ; Sharpe LJ; Brown AJ; Callaghan R; Gelissen IC, 2022, 'Corrigendum to “Regulation of ABCG4 transporter expression by sterols and LXR ligands” [PMID: 33141061, DOI: 10.1016/j.bbagen.2020.129769] (BBA - General Subjects (2021) 1865(1), (S0304416520302804), (10.1016/j.bbagen.2020.129769))', Biochimica et Biophysica Acta - General Subjects, 1866,

Journal articles | 2021

Brown AJ; Sharpe LJ; Rogers MJ, 2021, 'Oxysterols: From physiological tuners to pharmacological opportunities', British Journal of Pharmacology, 178, pp. 3089 - 3103,

Journal articles | 2021

Scott NA; Sharpe LJ; Brown AJ, 2021, 'The E3 ubiquitin ligase MARCHF6 as a metabolic integrator in cholesterol synthesis and beyond', Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1866,

Journal articles | 2021

Yang A; Alrosan AZ; Sharpe LJ; Brown AJ; Callaghan R; Gelissen IC, 2021, 'Regulation of ABCG4 transporter expression by sterols and LXR ligands', Biochimica et Biophysica Acta - General Subjects, 1865,

Journal articles | 2020

Capell-Hattam IM; Sharpe LJ; Qian L; Hart-Smith G; Prabhu AV; Brown AJ, 2020, 'Twin enzymes, divergent control: The cholesterogenic enzymes DHCR14 and LBR are differentially regulated transcriptionally and post-translationally', Journal of Biological Chemistry, 295, pp. 2850 - 2865,

Journal articles | 2020

Scott NA; Sharpe LJ; Capell-Hattam IM; Gullo SJ; Luu W; Brown AJ, 2020, 'The cholesterol synthesis enzyme lanosterol 14α-demethylase is post-translationally regulated by the E3 ubiquitin ligase MARCH6', Biochemical Journal, 477, pp. 541 - 555,

Journal articles | 2020

Sharpe LJ; Coates HW; Brown AJ, 2020, 'Post-translational control of the long and winding road to cholesterol', Journal of Biological Chemistry, 295, pp. 17549 - 17559,

Journal articles | 2020

Sharpe LJ; Coates HW; Brown AJ, 2020, 'Post-translational control of the long and winding road to cholesterol.', J Biol Chem, 295, pp. 17549 - 17559,

Journal articles | 2019

Brown A; Sharpe L, 2019, 'Oxysterols', Encyclopedia of Molecular Pharmacology, 3rd Edition, pp. 1 - 6,

Journal articles | 2019

Sharpe LJ; Howe V; Scott NA; Luu W; Phan L; Berk JM; Hochstrasser M; Brown AJ, 2019, 'Cholesterol increases protein levels of the E3 ligase MARCH6 and thereby stimulates protein degradation', Journal of Biological Chemistry, 294, pp. 2436 - 2448,

Journal articles | 2018

Aleidi SM; Yang A; Sharpe LJ; Rao G; Cochran BJ; Rye KA; Kockx M; Brown AJ; Gelissen IC, 2018, 'The E3 ubiquitin ligase, HECTD1, is involved in ABCA1-mediated cholesterol export from macrophages', Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1863, pp. 359 - 368,

Journal articles | 2017

Howe V; Sharpe LJ; Prabhu AV; Brown AJ, 2017, 'New insights into cellular cholesterol acquisition: promoter analysis of human HMGCR and SQLE, two key control enzymes in cholesterol synthesis', Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862, pp. 647 - 657,

Journal articles | 2017

Prabhu AV; Luu W; Sharpe LJ; Brown AJ, 2017, 'Phosphorylation regulates activity of 7-dehydrocholesterol reductase (DHCR7), a terminal enzyme of cholesterol synthesis', Journal of Steroid Biochemistry and Molecular Biology, 165, pp. 363 - 368,

Journal articles | 2017

Sharpe LJ; Brown AJ, 2017, 'Identifying sterol response elements within promoters of genes', , 1583, pp. 185 - 191,

Journal articles | 2016

Howe V; Sharpe LJ; Alexopoulos SJ; Kunze SV; Chua NK; Li D; Brown AJ, 2016, 'Cholesterol homeostasis: How do cells sense sterol excess?', Chemistry and Physics of Lipids, 199, pp. 170 - 178,

Journal articles | 2016

Loregger A; Cook E; Nelson J; Moeton M; Sharpe L; Engberg S; Karimova M; Lambert G; Zelcer N, 2016, 'A MARCH6 and IDOL E3 ubiquitin ligase circuit uncouples cholesterol synthesis from lipoprotein uptake in hepatocytes', Atherosclerosis, 252, pp. e250 - e250,

Journal articles | 2016

Loregger A; Cook ECL; Nelson JK; Moeton M; Sharpe LJ; Engberg S; Karimova M; Lambert G; Brown AJ; Zelcer N, 2016, 'A MARCH6 and IDOL E3 ubiquitin ligase circuit uncouples cholesterol synthesis from lipoprotein uptake in hepatocytes', Molecular and Cellular Biology, 36, pp. 285 - 294,

Journal articles | 2016

Luu W; Sharpe LJ; Capell-Hattam I; Gelissen IC; Brown AJ, 2016, 'Oxysterols: Old Tale, New Twists', Annual Review of Pharmacology and Toxicology, 56, pp. 447 - 467,

Journal articles | 2016

Prabhu AV; Luu W; Li D; Sharpe LJ; Brown AJ, 2016, 'DHCR7: A vital enzyme switch between cholesterol and vitamin D production', Progress in Lipid Research, 64, pp. 138 - 151,

Journal articles | 2016

Prabhu AV; Luu W; Sharpe LJ; Brown AJ, 2016, 'Cholesterol-Mediated Degradation of 7-Dehydrocholesterol Reductase Switches the Balance from Cholesterol to Vitamin D Synthesis', Journal of Biological Chemistry,

Journal articles | 2015

Aleidi SM; Howe V; Sharpe LJ; Yang A; Rao G; Brown AJ; Gelissen IC, 2015, 'The E3 ubiquitin ligases, HUWE1 and NEDD4-1, are involved in the post-translational regulation of the ABCG1 and ABCG4 lipid transporters', Journal of Biological Chemistry, 290, pp. 24604 - 24613,

Journal articles | 2015

Luu W; Hart-Smith G; Sharpe LJ; Brown AJ, 2015, 'The terminal enzymes of cholesterol synthesis, DHCR24 and DHCR7, interact physically and functionally', Journal of Lipid Research, 56, pp. 888 - 897,

Journal articles | 2015

Moon H; Ruelcke JE; Choi E; Sharpe LJ; Nassar ZD; Bielefeldt-Ohmann H; Parat MO; Shah A; Francois M; Inder KL; Brown AJ; Russell PJ; Parton RG; Hill MM, 2015, 'Diet-induced hypercholesterolemia promotes androgen-independent prostate cancer metastasis via IQGAP1 and caveolin-1', Oncotarget, 6, pp. 7438 - 7453,

Journal articles | 2015

Sharpe LJ; Howe V; Prabhu AV; Luu W; Brown AJ, 2015, 'Navigating the shallows and rapids of cholesterol synthesis downstream of HMGCR', Journal of Nutritional Science and Vitaminology, 61, pp. S154 - S156,

Journal articles | 2015

Sharpe LJ; Rao G; Jones PM; Glancey E; Aleidi SM; George AM; Brown AJ; Gelissen IC, 2015, 'Cholesterol sensing by the ABCG1 lipid transporter: Requirement of a CRAC motif in the final transmembrane domain', Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1851, pp. 956 - 964,

Journal articles | 2014

Brown A; Sharpe L; Loregger A; Cook E; Phan L; Stevenson J; Zelcer N, 2014, 'One E3 ligase targets two key control points in cholesterol synthesis (605.5)', The FASEB Journal, 28,

Journal articles | 2014

Luu W; Zerenturk EJ; Kristiana I; Bucknal MP; Sharpe LJ; Brown AJ, 2014, 'Signaling regulates activity of DHCR24, the final enzyme in cholesterol synthesi[s]', Journal of Lipid Research, 55, pp. 410 - 420,

Journal articles | 2014

Prabhu AV; Sharpe LJ; Brown AJ, 2014, 'The sterol-based transcriptional control of human 7-dehydrocholesterol reductase (DHCR7): Evidence of a cooperative regulatory program in cholesterol synthesis', Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1841, pp. 1431 - 1439,

Journal articles | 2014

Sharpe LJ; Burns V; Brown AJ, 2014, 'A Lipidomic Perspective on Intermediates in Cholesterol Synthesis as Indicators of Disease Status', Journal of Genetics and Genomics, 41, pp. 275 - 282,

Journal articles | 2014

Sharpe LJ; Cook ECL; Zelcer N; Brown AJ, 2014, 'The UPS and downs of cholesterol homeostasis', Trends in Biochemical Sciences, 39, pp. 527 - 535,

Journal articles | 2014

Tint GS; Pan L; Shang Q; Sharpe LJ; Brown AJ; Li M; Yu H, 2014, 'Desmosterol in brain is elevated because DHCR24 Needs REST for robust expression but REST is poorly expressed', Developmental Neuroscience, 36, pp. 132 - 142,

Journal articles | 2014

Zelcer N; Sharpe LJ; Loregger A; Kristiana I; Cook ECL; Phan L; Stevenson J; Brown AJ, 2014, 'The E3 ubiquitin ligase MARCH6 degrades squalene monooxygenase and affects 3-hydroxy-3-methyl-glutaryl coenzyme a reductase and the cholesterol synthesis pathway', Molecular and Cellular Biology, 34, pp. 1262 - 1270,

Journal articles | 2014

Zerenturk EJ; Sharpe LJ; Brown AJ, 2014, 'DHCR24 associates strongly with the endoplasmic reticulum beyond predicted membrane domains: Implications for the activities of this multi-functional enzyme', Bioscience Reports, 34, pp. 107 - 117,

Journal articles | 2013

Luu W; Sharpe LJ; Brown AJ, 2013, 'Protein tyrosine phosphatase inhibition down-regulates ligand-induced ABCA1 expression', Atherosclerosis, 228, pp. 362 - 369,

Journal articles | 2013

Luu W; Sharpe LJ; Gelissen IC; Brown AJ, 2013, 'The role of signalling in cellular cholesterol homeostasis', IUBMB Life, 65, pp. 675 - 684,

Journal articles | 2013

Sharpe LJ; Brown AJ, 2013, 'Controlling cholesterol synthesis beyond 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR)', Journal of Biological Chemistry, 288, pp. 18707 - 18715,

Journal articles | 2013

Zerenturk EJ; Sharpe LJ; Ikonen E; Brown AJ, 2013, 'Desmosterol and DHCR24: Unexpected new directions for a terminal step in cholesterol synthesis', Progress in Lipid Research, 52, pp. 666 - 680,

Journal articles | 2012

Burns V; Sharpe LJ; Gelissen IC; Brown AJ, 2012, 'Species variation in ABCG1 isoform expression: Implications for the use of animal models in elucidating ABCG1 function', Atherosclerosis, 226, pp. 408 - 411,

Journal articles | 2012

Dimitri A; Livermore N; Sharpe L; Gandevia SC; Mckenzie DK; Butler JE, 2012, 'CBT REDUCES RATINGS OF BREATHING DIFFICULTY IN RESPONSE TO EXTERNAL RESISTIVE LOADS IN PEOPLE WITH COPD', RESPIROLOGY, 17, pp. 32 - 32,

Journal articles | 2012

Gelissen IC; Sharpe LJ; Sandoval C; Rao G; Kockx M; Kritharides L; Jessup WK; Brown AJP, 2012, 'Protein kinase A modulates the activity of a major human isoform of ABCG1', Journal of Lipid Research, 53, pp. 2133 - 2140,

Journal articles | 2012

Kockx M; Dinnes DL; Huang K; Sharpe LJ; Jessup W; Brown AJ; Kritharides L, 2012, 'Cholesterol accumulation inhibits ER to Golgi transport and protein secretion: Studies of apolipoprotein E and VSVGt', Biochemical Journal, 447, pp. 51 - 60,

Journal articles | 2012

Luu W; Sharpe LJ; Stevenson J; Brown AJ, 2012, 'Akt acutely activates the cholesterogenic transcription factor SREBP-2.', Biochimica et Biophysica ACTA - Molecular Cell Research, 1823, pp. 458 - 464,

Journal articles | 2012

Sharpe LJ; Wong J; Garner B; Halliday GM; Brown AJ, 2012, 'Is seladin-1 really a selective Alzheimer's disease indicator?', Journal of Alzheimer's Disease, 30, pp. 35 - 39,

Journal articles | 2012

Zerenturk EJ; Sharpe LJ; Brown AJ, 2012, 'Sterols regulate 3β-hydroxysterol Δ24-reductase (DHCR24) via dual sterol regulatory elements: Cooperative induction of key enzymes in lipid synthesis by sterol regulatory element binding proteins.', Biochimica et Biophysica ACTA - Molecular and Cell Biology of Lipids, 1821, pp. 1350 - 1360,

Journal articles | 2011

Sharpe LJ; Eden J-S; White PA; Brown AJ, 2011, 'Norovirus RNA-dependent RNA polymerase is phosphorylated by an important survival kinase, Akt', Journal of Virology, 85, pp. 10894 - 10898,

Journal articles | 2011

Sharpe LJ; Luu W; Brown AJ, 2011, 'Akt phosphorylates Sec24: New clues into the regulation of ER-to-Golgi trafficking', Traffic (Malden), 12, pp. 19 - 27,

Journal articles | 2010

Kristiana I; Sharpe LJ; Catts VS; Lutze-mann LH; Brown AJ, 2010, 'Antipsychotic drugs upregulate lipogenic gene expression by disrupting intracellular trafficking of lipoprotein-derived cholesterol.', Journal of Pharmacogenomics, 10, pp. 396 - 407

Journal articles | 2010

Krycer JR; Sharpe LJ; Luu W; Brown AJ, 2010, 'The Akt-SREBP nexus: cell signaling meets lipid metabolism.', Trends in Endocrinology and Metabolism, 21, pp. 268 - 278

Journal articles | 2008

Sharpe LJ; Brown AJ, 2008, 'Rapamycin down-regulates LDL-receptor expression independently of SREBP-2', Biochemical and Biophysical Research Communications, 373, pp. 670 - 674

Journal articles | 2008

Sharpe LJ; Du X; Brown AJ, 2008, 'Approaches to investigate the role of signaling in ER-to-golgi transport', Methods in Molecular Biology, 457, pp. 215 - 225,
Conference Abstracts | 2022

Capell-Hattam IM; Fenton NM; Sharpe LJ; Brow AJ, 2022, 'A new kid in the Bloch? The non-catalytic protein ERG28 plays a functional role in mammalian cholesterol synthesis and is co-regulated transcriptionally', in FEBS OPEN BIO, WILEY, Vol. 12, pp. 267 - 267,

Conference Abstracts | 2020

Capell-Hattam I; Sharpe L; Qian L; Hart-Smith G; Prabhu A; Brown A, 2020, 'Twin enzymes, divergent control: The cholesterogenic enzymes DHCR14 and LBR are differentially regulated transcriptionally and post-translationally', in FASEB JOURNAL, WILEY, CA, San Diego, Vol. 34, presented at Annual Meeting on Experimental Biology, CA, San Diego, 04 April 2020 - 07 April 2020,

Conference Abstracts | 2020

Scott N; Sharpe L; Capell-Hattam I; Gullo S; Luu W; Brown A, 2020, 'The Cholesterol Synthesis Enzyme Lanosterol 14 alpha-Demethylase is Post-Translationally Regulated by the E3 Ubiquitin Ligase MARCH6', in FASEB JOURNAL, WILEY, CA, San Diego, Vol. 34, presented at Annual Meeting on Experimental Biology, CA, San Diego, 04 April 2020 - 07 April 2020,

Conference Papers | 2016

Sharpe LJ; Prabhu AV; Luu W; Brown AJ, 2016, 'Cholesterol-Mediated Degradation of 7-Dehydrocholesterol Reductase Switches the Balance from Cholesterol to Vitamin D Synthesis', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, CA, San Diego, presented at Experimental Biology Meeting, CA, San Diego, 02 April 2016 - 06 April 2016,

Conference Abstracts | 2015

Luu W; Zerenturk E; Kristiana I; Sharpe L; Brown A, 2015, 'Signaling Regulates Activity of DHCR24, the Final Enzyme in Cholesterol Synthesis', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Vol. 29,

Conference Abstracts | 2015

Prabhu A; Luu W; Sharpe L; Brown A, 2015, 'The Interplay between Cholesterol and Vitamin D Biosynthesis', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, Vol. 29,

Conference Presentations | 2014

Moon H; Sharpe L; Choi E; Bielefeldt-Ohmann H; Nassar Z; Parat M-O; Francois M; Lee CS; Brown A; Russell P; Inder K; Hill M, 2014, 'Hypercholesterolemia promotes prostate cancer PC-3 metastases in orthotopic xenograft mice', presented at 105th Annual Meeting of the American-Association-for-Cancer-Research (AACR), CA, San Diego, 05 April 2014 - 09 April 2014,

Conference Papers | 2013

Luu W; Sharpe LJ; Stevenson J; Brown AJ, 2013, 'Signalling and cholesterol metabolism', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, MA, Boston, presented at Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB), MA, Boston, 20 April 2013 - 24 April 2013,

Conference Papers | 2013

Zerenturk E; Sharpe LJ; Kristiana I; Brown AJ, 2013, 'DHCR24: Unexpected new directions for a terminal step in cholesterol synthesis', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, MA, Boston, presented at Joint Annual Meeting of the ASPET/BPS at Experimental Biology (EB), MA, Boston, 20 April 2013 - 24 April 2013,

Understanding how cholesterol synthesis enzymes are regulated.

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